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The antibody is from purified rabbit serum by affinity purification through sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been observed in several tumors, which leads to chromosome instability, and thus may well contribute to tumorigenesis. This protein was found to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a essential function in promoting mobile-cycle progression in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, have been described. Two extra splice variants were being described but thorough nucleotide sequence facts just isn't yet out there.
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Blocking peptides are peptides that bind exclusively to the goal antibody and block antibody binding. These peptide commonly consists of the epitope acknowledged from the antibody.
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This manual outlines additional possible optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This guideline outlines more attainable optimization of mobile and immunoassay parameters to guarantee the best possible outcomes are...
Antibodies certain to the blocking peptide not bind for the epitope about the goal protein. By comparing the staining in the blocked antibody versus the antibody alone, you can see which staining is unique.
Concentrate on Info Cyclin E belongs for the very conserved cyclin household, whose associates are characterized by a remarkable periodicity in protein abundance through the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinctive cyclins show distinctive expression and degradation designs which contribute towards the temporal coordination of each and every mitotic celebration. Cyclin E kinds a complex with and capabilities for a regulatory subunit of CDK2, whose action is required for cell cycle G1/S transition. This protein accumulates at the G1-S section boundary which is degraded as cells progress as a result of S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by both antibodies...
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